By Xueji Zhang, Haifeng Dong, Yaping Tian
This e-book summarizes microRNA (miRNA) biology in a number of pathological approaches, emphasizing the numerous strength purposes of miRNA in diagnostics and prognostics, in addition to novel drug goals. the traditional ideas used for miRNA detection together with common PCR, Northern blotting, microarray and clone equipment are addressed. fresh rising suggestions in miRNA detection and quantification with more desirable flexibility and suppleness, similar to novel molecular organic suggestions and locked nucleic acid (LNA) converted probes, in addition to nanotechnology-based methods, also are incorporated. The booklet additionally highlights the newest advances in clinical-related miRNA detection tools in dwelling cells, circulating blood and tissue, resembling in situ hybridization (ISH) and molecular imaging strategies, that are beneficial to explain the biogenesis and organic functionality of miRNAs in vivo. eventually, the respective merits and downsides of varied detection innovations during this fast-moving box are mentioned, in addition to the demanding situations and promising new directions.
This e-book deals a important source for analytical chemists, biologists and physicians excited by miRNA research.
Dr. Xueji Zhang and Dr. Haifeng Dong are Professors on the tuition of Chemistry & organic Engineering, college of technological know-how & expertise Beijing (USTB), China.
Dr. Yaping Tian is a Professor on the division of scientific Biochemistry, chinese language PLA normal health facility and armed forces scientific college, China.