By Ralph I. Dorfman (Eds.)
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Summing totals over each pair of factors, w e get, among the completely hypophysectomized survivors: Living mice Effect Effect Effect Effect of of of of 23:51 32:42 36:38 36:38 anesthesia age temperature glucose T h e data were converted to angles t o equalize variances, the analysis of variance is shown in T a b l e X X I I I and confirms the conclusions a b o v e . T h e suspicion of an age effect is seen not to be confirmed. 50 C. W . 0 — ° Data in Table X X I (complete hypophysectomy). From Lamond and Emmens (1959).
W h e n e v e r the test objects can be subdivided into several groups believed to be more homogeneous than the whole, or whenever a test object can be used more than once, various restrictions in the design of assays become possible, which will usually contribute materially t o the reduction of error. I t m a y also be possible to give different doses t o different animals in the same subclass, after preliminary knowledge of individual response levels (Bliss and M a r k s , 1939b; Claringbold, 1 9 5 5 ) .
Modified from Emmens (1957). 1. 0 — " Data in Table X V I I , determined from group responses. From Emmens (1957). 05. 001. period, although the data of Emmens (1939) indicated that they would, and second, t o o m a n y 0 or 1 0 0 % responses might occur. T h e chance o f this was, however, minimized in the design of the test since each mouse was used eight times. These tests were n o t assays, because they investigated the slope differences between estrogens and proestrogens (Emmens, 1941), but they were in all other respects of the same design as an assay w o u l d be, were slopes the same.