By P. B. Gahan
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A lot of our wisdom of the cerebral stream has been derived from reviews of species except human. there's increas ing attractiveness of species variations and main issue that experiences in animals might be deceptive if unquestioningly utilized to the human. A dramatic instance of this happened within the early his tory of the learn of the circulate of the mind.
Although the quantity covers 22 papers via 36 authors from 12 nations, the heritage within the historical past is certain to Hungary the place, in 1973 Andras Pn§kopa began to lay the basis of a systematic discussion board, that are a customary assembly spot for specialists of the area within the box. due to the fact then, there was a relentless curiosity in that discussion board.
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Extra info for Autoradiography for Biologists
Histochem. , 14, 414. (1967). J. Roy. Micr. Soc, 87, 489. (1968). , 8, 115. A P P L E T O N , T . C , P E L C , S . , a n d T A R B I T , M . H . (1969). J. , 5, 4 5 . B I T E N S K Y , L . , S I L C O X , Α . Α . , a n d C H A Y E N , J . (1965). J. Roy. Micr. Soc, 84, 397. G A H A N , P . B . , a n d R A J A N , A . K . ( 1 9 6 6 ) . ^ . Exp. , 17, 34. G A H A N , P . , M C L E A N , J . , K A L I N A , M . , a n d S H A R M A , W . (1967). J. Exp. , 18, 151. M E R R Y M A N , Η . T . (1956).
F r e e z i n g or Q u e n c h i n g t h e T i s s u e s W h e n a tissue is frozen, cellular and extra-cellular water usually freeze at different rates so that depending on the speed of freezing ice crystals are formed (Merryman, 1950, 1960). I n the ideal physical state where only water is present vitreous ice is formed with rapid freezing (Pryde and Jones, 1957), b u t it is doubtful whether this ever happens in biological material where there is a rapidly changing complex of solutes. T h e tissue is damaged by the formation of large ice crystals to an extent which makes sectioning difficult or impossible and which results in a distortion of the subsequent histological detail.
Sectioning. See description for animal material. Smears or squashes Smears or squashes can be effectively used for autoradiography. Preparations m a d e by any of the standard techniques are fixed and washed, and then set u p for autoradiography as described for sections. IV. S e t t i n g u p A u t o r a d i o g r a p h s If sections from e m b e d d e d material are used it is necessary to remove all of the wax before the application of photographic emulsion. For smears and squashes, proceed straight to "Application of emulsion".