Download Analytical Chemistry of Bacillus thuringiensis by Leslie A. Hickle, William L. Fitch PDF

By Leslie A. Hickle, William L. Fitch

content material: Analytical chemistry of Bacillus thuringiensis : an outline / Leslie A. Hickle and William L. Fitch --
historic points of the quantification of the energetic element percent for Bacillus thuringiensis items / George Tompkins, Reto Engler, Michael Mendelsohn, and Phillip Hutton --
Bioassay tools for quantification of Bacillus thuringiensis [delta]-endotoxin / Clayton C. Beegle --
Specificity of insecticidal crystal proteins : implications for commercial standardization / R. Milne, A.Z. Ge, D. Rivers, and D.H. Dean --
In vitro analyses of Bacillus thuringiensis [delta]-endotoxin motion / George E. Schwab and Paul Culver --
id of entomocidal pollutants of Bacillus thuringiensis via high-performance liquid chromatography / Takashi Yamamoto --
Characterization of parasporal crystal pollutants of Bacillus thuringiensis Subspecies kurstaki lines HD-1 and NRD-12 : use of oligonucleotide probes and cyanogen bromide mapping / L. Masson, M. Bossé, G. Préfontaine, L. Péloquin, P.C.K. Lau, and R. Brousseau --
improvement of a high-performance liquid chromatography assay for Bacillus thuringiensis var. san diego [delta]-endotoxin / Leonard Wittwer, Denise Colburn, Leslie A. Hickle, and T.G. Sambandan --
Use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to quantify Bacillus thuringiensis [delta]-endotoxins / Susan M. Brussock and Thomas C. Currier --
Quantitative immunoassay of insecticidal proteins in Bacillus thuringiensis items / R. Gene Groat, James W. Mattison, and Eric J. French --
The light-scattering characterization of [delta]-endotoxin creation in inclusion our bodies / Fritz S. Allen, Betty J.M. Hannoun, Tammy B. Hebner, and Kathryn Nette --
Quantification of Bacillus thuringiensis insect keep watch over protein as expressed in transgenic crops / Roy L. Fuchs, Susan C. MacIntosh, Duff A. Dean, John T. Greenplate, Frederick J. Perlak, Jay C. Pershing, Pamela G. Marrone, and David A. Fischhoff --
High-performance liquid chromatography research of 2 [beta]-exotoxins produced by way of a few Bacillus thuringiensis traces / Barry L. Levinson.

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Extra resources for Analytical Chemistry of Bacillus thuringiensis

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Much of the progress which resulted in the areas of molecular genetics and cell biology of B. thuringiensis mode of action can be traced to the international cooperative effort led by Howard Dulmage, Denis Burges and Hugette deBarjac and sponsored by the World Health Organization (7). This effort led to considerable information on strain specificity [most of which has yet to be published] and perhaps more importantly to a set of strains, the HD collection, which has become a tremendous resource for genetic, biochemical and bioassay data.

Biochem. J. 1989, 260, 87-91. Haider, M. ; Ellar, D. J. Biochem. J. 1987, 248, 197-201. ; Vaek, M. Eur. J. Biochem. 1986, 161, 278-80. ; Pliska, V. Eur. J. Biochem. 1988, 156, 531-40. ; VanMellaert, H. Eur. J. Biochem. 1989, (in press) Wolfersberger, M. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. Chapter 5 In Vitro Analyses of Bacillus thuringiensis δ-Endotoxin Action George E. ) endotoxins as determined by in vitro experimentation. t. endotoxins. The use of cell culture and isolated tissue preparations, which comprise the primary model systems for in vitro experimentation, has certain limitations.

Location of Specificity Domains. Because of the lack of knowledge about the structure of B. thuringiensis insecticidal crystal proteins, past discussions about the specificities of crystal proteins tend to emphasize host processes which act upon crystal proteins rather than intrinsic properties of proteins themselves. Aside from host factors such as insect midgut proteases, pH, and types of crystal protein toxin receptors, the specificities of insecticidal crystal proteins seem to be determined by amino acid sequences located on the variable domains of these proteins.

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